Invicro’s ex vivo imaging team has expertise in histology, IHC, immunofluorescence, quantitative whole-body autoradiography (QWBA), microautoradiography (MARG) and receptor binding assays, among other techniques. Our histology facilities are fully-equipped with three cryostats, two whole-body cryomacrotomes for frozen sections, and four microtomes for cutting paraffin embedded tissue. Notably, we have developed a method termed Cryo-Fluorescence Tomography (CFT) that enables 3D visualization of fluorescent compound distribution in frozen tissue. We have expertise in IHC custom stain development, double or triple labeling on frozen and paraffin sections.

Our ex vivo imaging capabilities are often included at an endpoint in in vivo studies to provide high-resolution imaging, further expanding the value and information obtained per sample. This is achieved in a multi-modality approach in which the same pre-clinical sample is imaged whole using CFT and/or autoradiography to provide 3D high resolution information throughout the sample. Ex vivo 3D images are than co-registered with in vivo scans. Further tissue collection of sections from the same sample allows for microscopical examination thus providing cellular resolution. Our multi-modality approach together with our complementary ex vivo capabilities provide a unique opportunity to generate temporal and spatial information from low to high resolution in the same sample.

We have experience in many therapeutic areas including: oncology, neuroscience, bone, ocular, renal, inflammation, and cardiovascular. Invicro specializes in the development of novel image analysis methods, leveraging VivoQuant and other internal tools for the quantification of image features from digitized slides.

  • Mouse Tumor CD31

    Rat kidney PAM stain with nuclear red counterstain

    Mouse tumor PCNA (blue) and Caspase-3 (brown)

    Mouse Pancreas Insulin (Red) and Glucagon (Dark Blue)

    Rabbit femur MMA section (Goldner’s trichrome)

    Mouse spleen CD3

  • IHC Stain list IHC Double/Triple Stain
    Alpha Smooth Muscle Actin Cytokeratin 18 Macrophage PCNA and Caspase 3
    Aggrecan Desmin MAC 387 Wilms Tumor-1 and Desmin
    Apelin Receptor (APJ) Double Cortin MAP-2 CD31 and Desmin
    Beta-Amyloid Fibronectin NCAM (CD56) CD31 and LYVE-1
    BMP-2 F4/80 NF-200 CD31 and alpha-SMA
    CA9 GAP43 NIMP CD31 and Guanylate Cyclase a1
    Caspase-3 GFAP PCNA CD31 and Guanylate Cyclase β1
    CD274 (PD-L1) GLEPP1 PEG CD90 and Guanylate Cyclase a1
    CD3 Guanylate Cyclase a1 PGP 9.5 CD90 and Guanylate Cyclase β1
    CD11b Guanylate Cyclase β1 phospho-Rb Insulin and Glucagon
    CD31 Glucagon RECA RECA and alpha-SMA
    CD34 Granulocyte s100A8 RECA-1 and LYVE-1
    CD4 Gr-1 sGC-α1 RECA-1 LYVE-1 and Collagen III
    CD40 HIF-1a sGC-β1 RECA-1 LYVE-1 and Alpha-SMA
    CD45R Human Mitochondria Tyrosine Hydroxylase
    CD68 IgA TIM-1/KIM-1
    CD90 IgG VEGF
    C-Fos IL-8Rb VEGF-C
    Collagen I IBA-1 VEGF-D
    Collagen II Insulin Vimentin
    Collagen III Ki67 Von Willebrand Factor
    Collagen IV LC3 Wilms tumor-1
    Cytokeratin 7 LYVE-1
  • Routine Stain List
    Alcian Blue-PAS-hematoxylin stain PAS
    Alkaline Phosphatase stain Periodic acid-methenamine-silver stain (PAM)
    Aniline Blue stain Picrosirius red/van gieson picrofuchsins stain
    Goldners Trichrome stain Safranin O/fast green stain
    Gomori's Trichrome stain Stevenal's blue stain
    Gram stain Sanderson's rapid bone stain
    H&E Sirius red stain
    Luxol fast blue and PAS stain Toluidine blue Stain
    Masson's Trichrome stain TRAP stain
    Methylene blue/basic fuchsin stain TUNEL stain
    Movat pentachrome stain Villanueva bone stain
    Nissl stain Von kossa stain